استخراج و خنثی‌سازی سم نماتوسیست عروس دریایی Crambionella orsini با استفاده از کیلیت Na-EDTA

نوع مقاله: مقاله پژوهشی

نویسندگان

1 1. گروه شیمی دریا، دانشکده علوم دریایی و اقیانوسی، دانشکاه علوم و فنون دریایی خرمشهر، خرمشهر

2 موسسه واکسن و سرم سازی رازی

چکیده

عروس دریایی از جمله جانوران زهرآگینی است که سم آن موجب مسمومیت انسان می‌گردد. گونه‌های متنوعی از عروس دریایی در خلیج‌فارس یافت می‌شود. این گونه‌ها اگرچه موجب مرگ سریع انسان نمی‌گردند اما اثرات سوئی بر سیستم جسمانی انسان داشته و عوارض جانبی در پی دارند. در این تحقیق سم عروس دریایی Crambionella Orsini طبق روش Bloom استخراج‌شده و غلظت آن توسط روش Biuret و 50LD سم طبق روش Jung and Choi بدست آمد. با توجه به غلظت سم و50LD آن مشخص شد که ml5/0 از سم موجب مرگ موش می‌گردد. از Na-EDTA برای خنثی کردن سم استفاده شد. این کیلیت به دو طریق به موش تزریق گردید که در هر دو روش مانع مرگ شد. Na-EDTA یک کیلیت اختصاصی برای دفع کلسیم از بدن می‌باشد که با توجه به هسته کلسیمی سم قادر است آن را از ساختار سم جدا کرده و موجب خنثی شدن سم گردد.

کلیدواژه‌ها

موضوعات


A. G., & Lieberman, B. S. (2007). Exceptionally preserved jellyfishes from the Middle Cambrian. PloS one, 2(10), e1121.

Ceren, C. R., & Odell, G. V. (1984). Biochemistry of spider venoms.Handbook of natural toxins.

Cohen, B. D., Spritiz, N., Lubash, G. D., & Rubin, A. L. (1959). Use of a calcium chelating agent (NaEDTA) in cardiac arrhythmias. Circulation,19(6), 918-927.

Collins, S. P., Comis, A., Marshall, M., Hartwick, R. F., & Howden, M. E. H. (1993). Monoclonal antibodies neutralizing the haemolytic activity of box jellyfish (Chironex fleckeri) tentacle extracts. Comparative Biochemistry and Physiology Part B: Comparative Biochemistry106(1), 67-70.

Crisponi, G., Nurchi, V. M., Lachowicz, J. I., Crespo-Alonso, M., Zoroddu, M. A., & Peana, M. (2015). Kill or cure: Misuse of chelation therapy for human diseases. Coordination Chemistry Reviews284, 278-285.

Daly, M., Brugler, M. R., Cartwright, P., Collins, A. G., Dawson, M. N., Fautin, D. G., ... & Romano, S. L. (2007). The phylum Cnidaria: a review of phylogenetic patterns and diversity 300 years after Linnaeus.

Endean, R., Duchemin, C., McColm, D., & Fraser, E. H. (1969). A study of the biological activity of toxic material derived from nematocysts of the cubomedusan Chironex fleckeri. Toxicon6(3), 179-204.

Fujii, H., Kurahshi, T., cong, Z., Ohtsuki, A., Wang, C. and Tanizawa, M., 2011. Structure-Function relationship of metalloproteins. Annual Review. 80–81.

Hadavand, 1393. Extraction and identification of the Ghetto Seaweed Nematocytic Poison. Marine Science and Technology University of Khorramshahr. MSc Thesis. P. 56

Haddock, S. H. D., & Case, J. F. (1999). Bioluminescence spectra of shallow and deep-sea gelatinous zooplankton: ctenophores, medusae and siphonophores. Marine Biology133(3), 571-582.

Holstein, T. and Tardent, P., 1984. An ultrahigh-speed analysis of exocytosis: nematocyst discharge. Science, 223 (4638): 830–833.

Houck, H. E., Lipsky, M. M., Marzella, L., & Burnett, J. V. (1996). Toxicity of sea nettle (Chrysaora quinquecirrha) fishing tentacle nematocyst venom in cultured rat hepatocytes. Toxicon, 34(7), 771-778.

 

Hsieh, Y. P., & Rudloe, J. (1994). Potential of utilizing jellyfish as food in Western countries. Trends in Food Science & Technology5(7), 225-229.

Jung, H., & Choi, S. C. (1994). Sequential method of estimating the LD50 using a modified up-and-down rule. Journal of biopharmaceutical statistics,4(1), 19-30.

Kass-Simon G., Scappaticci A. A., 2002. The behavioral and developmental physiology of nematocysts. Canadian Journal of Zoology, 80: 1772–1794.

Kawabata, T., Lindsay, D. J., Kitamura, M., Konishi, S., Nishikawa, J., Nishida, S., ... & Nagai, H. (2013). Evaluation of the bioactivities of water-soluble extracts from twelve deep-sea jellyfish species. Fisheries Science,79(3), 487-494.

Keen, T. E. B. (1971). Comparison of tentacle extracts from Chiropsalmus quadrigatus and Chironex fleckeri. Toxicon9(3), 249-254.

Kim, E., Lee, S., Kim, J. S., Yoon, W. D., Lim, D., Hart, A. J., & Hodgson, W. C. (2006). Cardiovascular effects of Nemopilema nomurai (Scyphozoa: Rhizostomeae) jellyfish venom in rats. Toxicology letters167(3), 205-211.

Kreil, G. (1995). Hyaluronidases-a group of neglected enzymes. Protein science: a publication of the Protein Society4(9), 1666.

Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. nature227(5259), 680-685.Lee, H., Jung, E. S., Kang, C., Yoon, W. D., Kim, J. S., & Kim, E. (2011). Scyphozoan jellyfish venom metalloproteinases and their role in the cytotoxicity. Toxicon58(3), 277-284.

Mostafaei, AS, 1378. Practical and theoretical guide for protein electrophoresis in gel. Tazkeyh Publishing House, Tehran, No. 1. The first year., P. 127.

Nget-Hong, T., & Gnanajothy, P. (1992). Comparative study of the enzymatic, hemorrhagic, procoagulant and anticoagulant activities of some animal venoms. Comparative Biochemistry and Physiology Part C: Comparative Pharmacology103(2), 299-302.

Oviedo, C., & Rodríguez, J. (2003). EDTA: the chelating agent under environmental scrutiny. Quimica Nova, 26(6), 901-905.

Ozacmak, V. H., Thorington, G. U., Fletcher, W. H., & Hessinger, D. A. (2001). N-acetylneuraminic acid (NANA) stimulates in situ cyclic AMP production in tentacles of sea anemone (Aiptasia pallida): possible role in chemosensitization of nematocyst discharge. Journal of Experimental Biology204(11), 2011-2020.

Purves, W. K., Sadava, D., Orians, G. H. and Heller, H. C., 1998. Life. The Science of Biology, 4: The Evolution of Diversity. Chapter 31.

Ramasamy, S., Isbister, G. K., Seymour, J. E., & Hodgson, W. C. (2005). The in vivo cardiovascular effects of an Australasian box jellyfish (Chiropsalmus sp.) venom in rats. Toxicon45(3), 321-327.

Satterlie, R. A. (2002). Neuronal control of swimming in jellyfish: a comparative story. Canadian Journal of Zoology80(10), 1654-1669.

Takeya, H., Onikura, A., Nikai, T., Sugihara, H., & Iwanaga, S. (1990). Primary structure of a hemorrhagic metalloproteinase, HT-2, isolated from the venom of Crotalus ruber ruber. Journal of biochemistry, 108(5), 711-719.

Tibballs, J. (2006). Australian venomous jellyfish, envenomation syndromes, toxins and therapy. Toxicon48(7), 830-859.

Turner, R. J., & Freeman, S. E. (1969). Effects of Chironex fleckeri toxin on the isolated perfused guinea pig heart. Toxicon7(4), 277-286.

Waldron, K. J., Rutherford, J. C., Ford, D., & Robinson, N. J. (2009). Metalloproteins and metal sensing. Nature460(7257), 823-830.

Zezima, K., 2010, Death Does Not Deter Jellyfish Sting, The New York Times, July 22, 2010, page A11.

Zhang, Z. Q. (2011). Animal biodiversity: An introduction to higher-level classification and taxonomic richness. Zootaxa, 3148, 7-12.