تأثیر عصاره آنزیمی باسیلوس‌های پروبیوتیکی بررشد و ترکیبات بیوشیمیایی لاشه لارو قزل‌آلای رنگین‌کمان (Oncorhynchus mykiss)

نوع مقاله: مقاله پژوهشی

نویسندگان

1 گروه شیلات، دانشکده منابع طبیعی، دانشگاه گنبد کاووس، گنبد کاووس، ایران

2 گروه علوم بیومدیکال، دانشگاه الزهرا، تهران، ایران

3 گروه علوم محیطی و بوم‌شناسی دریایی، دانشکده علوم زیستی دریا، دانشگاه اقیانوسی چین، چینگ‌تائو، چین

چکیده

مطالعه حاضر به منظور ارزیابی تأثیرعصاره آنزیمی تولید شده به وسیله پنج گونه باسیلوس پروبیوتیکی بر رشد و ترکیبات بیوشیمیایی لاشه لارو‌های ماهی قزل‌آلای رنگین‌کمان (Oncorhynchus mykiss) با وزن اولیه 197/0 ± 583/0 گرم صورت گرفت. عصاره آنزیمی توسط باسیلوس‌های پروبیوتیکی در محیط کشت مایع با غلظت‌های106 × 1، 107 × 1و 108 × 1 واحد کلنی در 100 گرم محیط کشت تولید شد و به ترتیب به جیره‌های لارو‌های ماهی قزل‌آلای رنگین‌کمان افزوده شد. نتایج بدست آمده از زیست‌سنجی لاروها در انتهای دوره مطالعه نشان داد که استفاده از سوسپانسیون آنزیمی با بیشترین غلظت میکروبی (108 × 1 واحد کلنی در 100 گرم) در تیمار سوم (77/3 ± 02/20 گرم) باعث افزایش معنی‌دار رشد گردیده است (05/0P

کلیدواژه‌ها


Alizadeh, M., Farzanfar, A. and Nafisi-Bahabadi, M. 2011. The effect of probiotic Bioplus 2B on growth performance and carcass composition of rainbow trout, Oncorhynchus mykiss (Walbaum, 1792) larvae. Indian Journal of Fisheries 58(4): 55-59.

AOAC. 1990. In: W. Horwitz (Ed.), Official Methods of Analyses, 15th edition. Association of Official Analytical Chemists Inc, Arlington, VA. P: 445.

Ayoleke, E.O., Greg, I.P., Morenike, A.A. and Dominique, P.B. 2006.Dietary incorporation of soybean meal and exogenous enzyme cocktail can affect physical characteristics of faecal material egested by rainbow trout (Oncorhynchus mykiss). Aquaculture 254:466-475.

Bairagi, A., Ghosh, K.S., Sen, S.K. and Ray, A.K. 2002. Enzyme producing bacterial floraisolated from fish digestive tracts. Aquaculture International 10: 109-121.

Boonyaratpalin, M., Promkunthong, W. and Hunter, B. 2000. Effect of enzyme Pretreatment on in vitro Glucose solubility of Asian Plant By product and Growth Digestibility of oil Palm Expeller Meal by Oreochromis Niloticus (Nile Tilapia). In Hartngsveldt W.V. (Ed). Proceeding the Third European symposium on feed enzymes, PP: 86-92.

Carter, C.G., Houlihan, D.F., Buchanan, B. and Mitchell, A.I. 1994. Growth and feed utilization efficiencies of seawater Atlantic salmon, Salmo salar L., fed a diet containing supplementary enzymes. Aquaculture and  Fishery Management 25: 37-46.

Dabrowski, K.R. and Glogowski, J. 1977. Studies on the role of exogenous proteolytic enzymes indigestion processes in fish. Hydrobiologia 54:129–134.

De Silva, S.S., and Anderson, T.A. 1995. Fish Nutrition in Aquaculture, Chapman & Hall, 2-6 Boundary Row, London SE1 8HN, UK, PP: 318.

 

Diab, A.S., EL-Nagar, O.G. and Abd-El-Hady, M.Y. 2002. Evaluation of Nigella sativa L. (black seeds; baraka), Allium sativum (garlic) & Biogen as a feed additives on growth performance of Oreochromis niloticus fingerlings. Veterinary Medicine Journal of Suez canal university 2: 745-753.

Douillet, P.A. and Langdon, C.J. 1994.Use of a probiotic for the culture of larvae of the pacific Oyster Crassostrea gigas (Thunberg). Aquaculture 119: 25-40.

Farhangi, M., Carter, C. 2007. Effect of enzyme supplementation to dehulled lupin-based diets on growth, feed efficiency, nutrient digestibility and carcass composition of rainbow trout, Oncorhynchus mykiss (Walbaum). Aquaculture Research 38 (12) : 1274-1282

Felix, N., and Sudharsan, M., 2004 . Effect of glycine betaine, a feed attractant affecting growth and feed conversion of juvenile fresh water prawn Macrobrachium rosenbergii. Aquaculture Nutrition 10:193-197.

Gatesoupe, F.J. and Ringo, E. 1998. Lactic acid bacteria in fish: a review. Aquaculture 199: 25-40.

Ghobadi, Sh., Matin-Far, A., Nezami, Sh.A. and Soltani, M. 2009.Influence of supplementary enzymes Avizyme on fish meal replacement by soy bean meal and its effects on growth performance and survival rate of rainbow trout (Oncorhynchus mykiss). Journal of Fisheries, 2:11-22.

Ghosh, K., Sen, S.K. and Ray, A.K. 2002a. Characterization of Bacillus Isolated from the Gut of Rohu, labeo rohita, fingerlings and its significance in digestion. Applied Aquaculture 12: 33-42.

Ghosh, K., Sen, S.K. and Ray, A.K. 2002b. Characterization of Bacillus Isolated from the Gut of Rohu, labeo rohita, fingerlings and its significance in digestion. Applied  Aquaculture 12: 33-42.

Ghosh, K., Sen, S.K. and Ray, A.K. 2004. Growth and survival of Rohu, Labeo rohita (Hamilton,1822) spawn feed diets fermented with intestine bacterium, Bacillus circulans. Acta Ichthyologica etpiscatoria 34(2):155-165.

Helland, S.J., Grisdale, H. B., and Nerland, S. 1996. A simple method for the measurement of daily feed intake of groups of fish in tanks. Aquaculture 139: 157-163.

Jafaryan, H. 2006. The effects of Bacillus bacteria as a probiotic on the growth factors, survival rate and digestive enzymes activity in the Persian sturgeon (Acipenser persicus) larvae by enrichment of Artemia urmiana nauplii”. Ph.D. Thesis of Fishery. Gorgan University of Agri. Sci. & Nat Res. Pp 103.

Jafaryan, H., Azari Takami, gh., Kamali, a., Soltanii, M. Habibi rezaei, M. 2007. Using  bioencapsulated probiotic bacteria in Artemia urmiana nauplii for growth and survival of Persian sturgeon (Acipenser persicus) larvae. Gorgan University of Agri. Sci. & Natural Resours. 14:77-87

Kar, N. and Ghosh, K. 2008. Isolation and Characterization of Extracellular Enzyme Producing Bacilli in the Digestive Tracts of Rohu, Labeo rohita (Hamilton) and Murrel, Channa punctatus (Bloch). Asian Fisheries Science 21:421-434.

Lara-Flores, M., Miguel, A., Beatriz, E. and Lopez-Madrid, W. 2003.Use of the bacteria Streptococcus faecium and Lactobacillus acidophilus, and the yeast Saccharomyces cerevisiae as growth promoters in Nile tilapia (Oreochromis niloticus). Aquaculture 216: 193-201.

Lin, S., Mai, K. Tan, B., 2007. Effects of exogenous enzyme supplementation in diets on growth and feed utilization in tilapia, Oreochromis niloticus. Aquaculture Research 38: 1645-1653.

Mohamadi-Azarrm, H., Abedian, A. and Abtahi, B. 2004. Effects of probiotic on growth and survival in rainbow trout (Oncorhynchus mykiss). Journal of Marine Science. 2-3: 69-75.

Mohamed, K.A. 2007.Effect of Using Probiotic and Yeast as Growth Promoters in Commercial Diet of Tilapia (Oreochromis niloticus) Fingerlings. Agricultural Research Journal. Suez Canal University 7 (3): 41-47.

Noveirian, H.A. and Nasrollahzadeh, A. 2012. The effects of different levels of biogen probiotic additives on growth indices and body composition of juvenile common carp (Cyprinus carpio L.).Caspian Journal of Environmental Science 10(1): 115-121.

Saha, S., Roy, R.N., Kumar-Sen, S. and Ray, A.K. 2006. Characterization of cellulase-producing bacteria from the digestive tract of tilapia, Oreochromis mossambica (Peters) and grass carp, Ctenopharyngodon idella (Valenciennes). Aquaculture Research 37: 380-388.

Sahandi, J., Jafaryan, H., Roozbehfar, R., Babaei, S. and Dehestani, M. 2012. The use of two enrichment forms (Brachionus plicatilis enrichment and rearing water enrichment) with probiotic bacilli spore on growth and survival of Silver carp (Hypophthalmichthys molitrix). Iranian Journal of  Veterinary Research 13(4): 289-295.

Sahandi, J. 2013. Effect of two probiotic, Bifidobacterium animalis and Bifidobacterium lactis in-feed on growth and digestibility of Rainbow trout larvae (Oncorhynchus mykiss). M.Sc. theses. University of Gonbad Kavous. Pp 106.

Soares, C.S., Filho, M.P., Roubach, R. et al. 2008. Protease exógena em dietas para juvenis de tucunaré-paca (Cichla sp.).  Revista Brasileira de Zootecnia 37(6) :971-976.

Shariatmadari, F., and Mohiti-Asl,. M. 2009. The additive of domestic animals, poultry and aquatic animals diets. Printed by University of Tarbiat Modarres, Tehran. Pp 417.

Yildirim, Y.B. and Turan, F. 2010. Effects of exogenous enzyme supplemention in diets on growth and feed utilization in African catfish, Clarias gariepinus. Journal of Animal and Veterinary Advances 9 (2): 327-331.

Zhou, A., Liu, Y., Shi, P., He, S., Yao, B. and Ringø, E. 2009. Molecular characterization of the autochthonous micro-biota in the gastrointestinal tract of adult yellow grouper (Epinephelus awoara) cultured in cages. Aquaculture 286: 184-189