Ahmad Imani; Mehrdad Farhangi; Gholamreza Rafiee; Razieh Yazdanparast; Isabel Vasquez Baanante
Volume 11, Issue 3 , November 2012, , Pages 4-15
Abstract
We collected 69 samples of Mudskipper from dominant spices periophthalmus waltoni for genetic study from Hendijan, Khore Zangi and Delvar. A specimen of fin was fixed in Ethanol alcohol (96%) and moved to Biotechnology lab in center study of Persian Golf in Bushehr. Total genomic DNA was extracted by ...
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We collected 69 samples of Mudskipper from dominant spices periophthalmus waltoni for genetic study from Hendijan, Khore Zangi and Delvar. A specimen of fin was fixed in Ethanol alcohol (96%) and moved to Biotechnology lab in center study of Persian Golf in Bushehr. Total genomic DNA was extracted by proteinase-K digestion, phenol: chloroform: isoamyl alcohol extraction and ethanol precipitation. The quality and quantity of DNA was assessed using 1% agarose gel electrophoresis and spectrophotomeric method. Polymerase chain reactions (PCR) were conducted on the target DNA using 6 primers RAPD. PCR products were separated by electrophoresis on polyacrylamid gels (8%) that were stained using silver nitrate. Allele frequency, the effective and real allele, expected and observed heterozygosis, genetic diversity, Shannon Information index, Gst and gene based on analysis of molecular variance (AMOVA) were calculated using the GENALEX and Pop Gene software. The average of Shannon index for Hendijan, Khore zangi and Delvar was respectively 0.3887, 0.2860 and 0.3509. Highest of Gst observed between Hendijan and Khore zangi (0.260) and lowest between Khore zangi and Delvar (0.195). Highest of gene flow (Nm) was observed between Delvar and Khore zangi (1.033) and lowest between Hendijan and Khore zangi (0.687). Highest of Genetic identity observed between Delvar and Khore zangi (0.924) but lowest was between Hendijan and Khore zangi community (0.833). Eventually this study show that three community of our research are separate and belong to two clusters.
Zoheir Shokouh Saljoghi; Akbar Malekpour; Majid Bakhtiary; Ahmad Imani; Mohammad Reza Ashrafzadeh; Mohammad Moheiseni
Volume 10, Issue 2 , September 2012, , Pages 69-78
Abstract
Water treatment in recirculation aquaculture systems (RAS) is one of the main methods in water quality management. In organic Ion exchange materials are used only for ammonia adsorption in rearing systems. Zeolites have negative charge in their surfaces and this property capable them to remove cations ...
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Water treatment in recirculation aquaculture systems (RAS) is one of the main methods in water quality management. In organic Ion exchange materials are used only for ammonia adsorption in rearing systems. Zeolites have negative charge in their surfaces and this property capable them to remove cations and metal ions. At this study by using a cationic surfactant (Tetradecyltrimethylamonium bromide) clinoptilolite surface for removing nitrogenous compound is changed. Studied zeolite was analysed by XRD and XRF. Results indicated that Heulandit was the main element in the sample. Experiment was done at three replicates under environmental conditions (temperature (10, 15 and 20) and pH (6, 6.5, 7, 7.5 and 8)). Results indicated that environmental factors influenced absorption capacity. Results showed that increased in temperature have significant effect on nitrate and nitrite removal by SMZ (P<0/05).Temperature cause Increasing in anion mobility and finally anion adsorption. pH have significant effect on nitrate adsorption (P<0/05) while any regular trend didn’t observe about nitrite. Increasing pH cause increase in ionization degree and adsorption. Results indicated that other factor such as anion concentration, existing other anions and counter ions impressed adsorption.