علوم زیستی دریا
Mahdie Amirinezhad; Narges Amrollahi Biuki; Morteza Yousefzadi; Arash Ghaderi
Abstract
This study investigated the green synthesis of cerium oxide nanoparticles using natural marine extracts. Characteristic peaks for CeO2 with X-ray diffraction spectrum to crystal planes (111), (200), (220) (311), (331), (420), and (422) are related. In the SEM images, the synthesized cerium oxide ...
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This study investigated the green synthesis of cerium oxide nanoparticles using natural marine extracts. Characteristic peaks for CeO2 with X-ray diffraction spectrum to crystal planes (111), (200), (220) (311), (331), (420), and (422) are related. In the SEM images, the synthesized cerium oxide nanoparticles are generally spherical with a size of about 10 to 12 nm. the FTIR analysis indicated the presence of prominent peaks that showed the presence of this nanoparticle in different capacity states of Ce +3, Ce4+ acts as an antioxidant on the surface of nano cerium. Nanoparticles synthesized from algae at concentrations of 1000, 500, and 250 µg /ml showed between 60-65% DPPH free radical inhibition. The highest reduction rate of macroalgae Sargassum ilicifolium at a concentration of 500 µg /ml, it is a significant difference between nanoparticles synthesized from the available extracts and tested at different concentrations. Antibacterial activity was observed (P≤0.05). It showed that Gram-negative bacteria are more resistant to CeO2 nanoparticles than Gram-positive bacteria. INTRODUCTIONToday, biological methods of nanoparticle synthesis using microorganisms, natural extracts, and nutrients have been suggested as suitable alternatives to chemical and physical methods (Das et al., 2013). Researchers have reported that cerium nanoparticles are effective in dealing with oxidative stress and have an antioxidant role, so these nanoparticles can act as a remover of various forms of reactive oxygen in many physiological and biochemical reactions in the body of living organisms (Dhall et al. al., 2017(. Microalgae are the main group of photoautotrophic organisms known as potential sources of secondary metabolites, phenolic compounds, pigments and polysaccharides. Biosynthesis targets algal secondary metabolites as reducing agents for stabilization of nanoparticles (NPs) (Barciela et al., 2022). Metabolites extracted from starfish with low molecular weight have remarkable characteristics. These isolated compounds include steroids, anthroquinones, alkaloids, phospholipids and peptides, which are a rich source of activity against microbes and have many uses in the field of medicine (Baharara et al., 2020). In this study, the antioxidant and antibacterial properties of green cerium oxide nanoparticles synthesized from marine extracts have been investigated. MATERIALS AND METHODS Sargassum ilicifolium macroalgae was collected from the coast of Chabahar and transported to the laboratory. Then the samples were washed several times with distilled water in order to separate the mud and dried in the shade and at a suitable temperature for a week and then ground to powder. Brittle star Ophiocoma scolopendrina was collected from Qeshm Island at the time of low tide and dried with a freeze dryer at -40C and then powdered with an industrial mill. In order to extract and synthesize CeO2NPs nano cerium oxide, 10 grams of powdered each of the samples was added to 100 ml of double distilled water and stirred for 3 to 4 hours at 60 degrees Celsius, then it was collected with filter paper and then Whitman. In an Erlenmeyer flask, 100 ml of 0.05 M of Ce(NO3)3.6H2O and 20 ml of distilled water were added to it and this solution was stirred using a magnetic stirrer until a homogeneous solution was formed (Altaf et al. al., 2021). Then 40 ml of the extract was added to the solution and the final volume was brought to 100 ml with distilled water and the reaction mixture was stirred on a hot magnetic plate with a temperature of 70-80 degrees Celsius for 3-4 hours. The formed particles after sufficient stirring time were centrifuged at 5000 rpm for 10 minutes, the nanoparticles were repeatedly washed with deionized water and dried in an oven at 60 degrees for 6 hours. Then, the obtained product was calcined in the oven at 400°C for 2 hours to produce a yellow powder of cerium oxide (Elahi et al., 2019). In the test of measuring the antioxidant capacity by the DPPH (2,2-diphenyl-1-picrylhydrazyl) radical method, concentrations of 1000, 500, 125, 250, 62.5 and 31.25 μg/ml were made from 10 mg/ml stock, from 100 μl of each concentration was taken and poured into 96-well plates in triplicate. Also, 100 microliters of 0.3 mM DPPH solution (394.3 g/mol) was added. The 96-well plates were placed in the dark for 30 minutes, and the absorbance of the samples was read by an Elizarider device at a wavelength of 517 nm. RESULTS In the FT-IR study, the peaks around 1417, 1575, and 1577, 1432 cm2 in samples A and B are related to carbonate compounds. Scanning electron microscopy (SEM) has shown that the nanoparticles are spherical and tend to aggregate.The highest reduction rate against ascorbic acid was observed in Sargassum macroalgae at a concentration of 500 μg/ml. DISCUSSION AND CONCLUSION The highest inhibitory activity of NPS synthesized for S. ilicifolium was observed (65.45 ± 0.5% at 1000 μg/ml). The basis of the biological activities of cerium nanoparticles is the Ce3+/Ce4+ surface ratio, the cycle of oxidation and reduction between the Ce3+ and Ce4+ states, which have a unique ability to absorb and release oxygen on their surface and play a key role in antioxidant activity (Dhall and Self, 2018).
علوم زیستی دریا
Sanaz Ahmadi Falehi; Ahmad Savari; Nasrin Sakhaei; Faedeh Amini; Babak Doustshenas
Abstract
Planktonic crustaceans are of specific importance in energy transfer due to their location in the middle links of the food chain of aquatic ecosystems. Therefore, they directly affect the abundance and diversity of the higher categories of the food pyramid, especially stocks and fishery resources. In ...
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Planktonic crustaceans are of specific importance in energy transfer due to their location in the middle links of the food chain of aquatic ecosystems. Therefore, they directly affect the abundance and diversity of the higher categories of the food pyramid, especially stocks and fishery resources. In this study, the impact of a tidal cycle on the zooplanktonic Crustacean ecosystem in the estuarine Bahmanshir River (Northwestern Persian Gulf) was studied. Our samples were collected during the spring of 2018 by plankton net with mesh sizes of 100 μm. Samples were taken at 5 stations to have a tidal effect on the abundance of zooplankton, approximately from 8:00 to 18:00 every two hours. In general, 72 species belonging to 28 families related to 3 groups of crustaceans named Cirripedia, Cladocera, and Copeoda were observed. The highest percentage of relative abundance was related to Cladocera with 35%, which was caused by the abundance of Daphnia mendotae. The highest diversity (Shannon index = 2.71 ± 0.04) of copepods was revealed to be caused by the large number of species at station 5 (near the mouth of the Bahmanshir River). Most species among the families belong to the Acartiidae family, which has 10 species of Acartia. The results of PCA analysis showed that among environmental factors, salinity has a direct effect on the total density, density of Harpacticoida and Calanoida, in the first component. The tides appear to significantly affect the density and types of planktonic crustaceans during the mentioned springtime periods. Our results showed relatively strong influence of the tidal cycle on the spatial pattern of Copepod assemblages in the study area. This study may be useful for future biological monitoring and seawater salinity intrusion into rivers in the northwest of the Persian Gulf.
علوم زیستی دریا
Adel Ghabtani; Seyed Mohammad Mousavi; Annahita Rezaie; Mohammad Zakeri; Isac Zamani
Abstract
The purpose of this study was to evaluate the effects of three commercial probiotics (Bio-Aqua®, Bio-Aqua pond®, and Multi Behsil 100) on the growth, nutrition, and biochemical parameters of common carp fingerlings. For this purpose, 210 common carp fingerlings (with a mean weight: 9.6±0.02 ...
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The purpose of this study was to evaluate the effects of three commercial probiotics (Bio-Aqua®, Bio-Aqua pond®, and Multi Behsil 100) on the growth, nutrition, and biochemical parameters of common carp fingerlings. For this purpose, 210 common carp fingerlings (with a mean weight: 9.6±0.02 g and mean length: 12.9±0.32 cm) were randomly divided into 7 treatments (treatment 1 (control): basic diet without probiotics, treatment 2: 0.2 g of Bio-Aqua®, treatment 3: 0.1 g of Bio-Aqua®, treatment 4: 0.002 g/L of Bio-Aqua pond® dissolved in tank water, treatment 5: 0.001 g/L Bio-Aqua pond ® dissolved in tank water, treatment 6: 0.2 g of Multi Behsil 100, and treatment 7: 0.1 g of probiotic Multi Behsil 100) in triplicates, and 10 fish in each replicate. The commercial probiotics were added to the commercial diet, and the fish were fed based on body weight percentage for 60 days. Based on the results, the use of probiotics Bio-Aqua® and MultiBehsil 100, caused positive effects on the growth and nutrition parameters of common carp, although the use of 0.1 g of Bio-Aqua® per kilogram of the diet of common carp led to an improvement in growth, although, increasing the amount of this probiotic at the level of 0.2 g/kg led to negative effects on growth performance. The use of Multibehsil 100, caused positive effects on common carp. Thus, the best effect on growth performance and body composition was seen in the diet containing MultiBehsil 100 (0.1 and 0.2 g/kg of basic diet). Therefore, it can be concluded that the use of MultiBehsil 100 and Bio-Aqua® have favorable effects on common carp.