علوم زیستی دریا
Maryam Hasani; Mahin Rigi; Moazameh Kordjazi; Shirin Hasani
Abstract
Omega-3 fatty acids play a role in achieving optimal health and protection against various diseases. Although the instability and oxidation of its essential fatty acids have limited its use in food products. Among the strategies used to avoid these challenges, the encapsulation method has been the most ...
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Omega-3 fatty acids play a role in achieving optimal health and protection against various diseases. Although the instability and oxidation of its essential fatty acids have limited its use in food products. Among the strategies used to avoid these challenges, the encapsulation method has been the most successful method. Therefore, the present study was performed with the aim of improving the stability of fish oil with encapsulation method and producing a useful product. In this research, the encapsulation of fish oil with a ratio of 3:1 (oil: coating) was done with malt dextrin, sodium casein ate, protein whey concentrates and modified starch (HICAP) by freeze drying. Yogurt enriched with encapsulated fish oil powder was produced and the physicochemical and sensory properties of the product were evaluated. The results showed that the particle size, particle dispersion index and encapsulation efficiency of the capsules were 0.85μm, 0.29 and 90%, respectively. Also, the results showed that the syneresis of yogurt enriched with microcapsules was lower and the water holding capacity was higher than the control and sample enriched with non-encapsulated fish oil. The increase of peroxide values in yogurt enriched with encapsulated fish oil had a slower trend than yogurt enriched with non-encapsulated oil. In all treatments, pH decreased and acidity increased. The sensory evaluation of the treatments revealed that the quality parameters of the yogurt treatments decreased during storage, and the yogurt treatments enriched with microencapsulated oil and the control treatment did not show any significant difference in terms of overall acceptance.
علوم زیستی دریا
Hassan Zadabbas Shahabadi; Arash Akbarzadeh; Hamideh Ofoghi; Sabihe Soleimanian-Zad; Saeid Kadkhodaei
Abstract
In modern aquaculture, energy consumption, raw materials used, and environmental effects should be considered, therefore, during the last decade, phytase has been used by aquatic feed industries as an enzyme supplement to increase the digestibility and absorption of plant compounds and improve growth ...
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In modern aquaculture, energy consumption, raw materials used, and environmental effects should be considered, therefore, during the last decade, phytase has been used by aquatic feed industries as an enzyme supplement to increase the digestibility and absorption of plant compounds and improve growth performance along with reducing phosphorus pollution in the aquatic environment. In this study, by using the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) system, the bacterial phytase gene was inserted into the model microalgae Chlamydomonas reinhardtii in the designed position of the NR gene, and the correct editing was checked and confirmed by PCR. Confirmation of the efficiency and expression of the phytase gene placed in the NR site was investigated using SDS Page gel. Also, the translation of the phytase gene was confirmed by SDS PAGE on five colonies containing the correct editing and one control sample colony as confirmation of the expression of the transgene inserted at the protein level, which indicated the successful transcription and translation of the phytase gene inserted in the exon 2 position of the microalgae NR gene. considering the benefits of producing and using phytase enzymes in aquatic diets, reducing production costs and eutrophication of aquatic ecosystems will be achieved and it can be a new perspective to accelerate the commercial production of other recombinant proteins in organisms with nutritional value "C. reinhardtii" and provide the development of the fish feed industry and modern aquaculture.
علوم زیستی دریا
Zahra Yarahmadi; Bita Archangi; Ahmad Savari; Mohammad Bagher Nabavi
Abstract
The objective of this study was to morphological description and phylogenetic study of Ornithocercus magnificus in Bandar Abbas waters. The genus Ornithocercus is a heterotrophic dinoflagellate of the family Dinophysaceae. The samples were collected using a phytoplankton net (mesh size: 20 µm) ...
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The objective of this study was to morphological description and phylogenetic study of Ornithocercus magnificus in Bandar Abbas waters. The genus Ornithocercus is a heterotrophic dinoflagellate of the family Dinophysaceae. The samples were collected using a phytoplankton net (mesh size: 20 µm) equipped with a flowmeter in surface waters. The plankton samples were scanned and identified with an inverted microscope. Then, the SSU rDNA gene fragment from individual cells have been amplified using the polymerase chain reaction and sequenced for the Iranian strain of this species. Phylogenetic analyzes were performed by Maximum Likelihood (ML) and Maximum parsimony (MP) methods. In this study, two new sequences of SSU rDNA from O. magnificus were reported for the first time and deposited in the GenBank. Morphological studies showed that the isolated cells from Iranian water were O. magnificus. Generally, the results showed; the morphological results of O. magnificus were matched with the molecular results of this study. Phylogenetic analysis grouped isolated Iranian sequences with O. magnificus and formed a monophyletic clad with other members of this genus as sister groups. The genus Histioneis is the closest relative to Ornithocercus.
علوم زیستی دریا
Mahdie Amirinezhad; Narges Amrollahi Biuki; Morteza Yousefzadi; Arash Ghaderi
Abstract
This study investigated the green synthesis of cerium oxide nanoparticles using natural marine extracts. Characteristic peaks for CeO2 with X-ray diffraction spectrum to crystal planes (111), (200), (220) (311), (331), (420), and (422) are related. In the SEM images, the synthesized cerium oxide ...
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This study investigated the green synthesis of cerium oxide nanoparticles using natural marine extracts. Characteristic peaks for CeO2 with X-ray diffraction spectrum to crystal planes (111), (200), (220) (311), (331), (420), and (422) are related. In the SEM images, the synthesized cerium oxide nanoparticles are generally spherical with a size of about 10 to 12 nm. the FTIR analysis indicated the presence of prominent peaks that showed the presence of this nanoparticle in different capacity states of Ce +3, Ce4+ acts as an antioxidant on the surface of nano cerium. Nanoparticles synthesized from algae at concentrations of 1000, 500, and 250 µg /ml showed between 60-65% DPPH free radical inhibition. The highest reduction rate of macroalgae Sargassum ilicifolium at a concentration of 500 µg /ml, it is a significant difference between nanoparticles synthesized from the available extracts and tested at different concentrations. Antibacterial activity was observed (P≤0.05). It showed that Gram-negative bacteria are more resistant to CeO2 nanoparticles than Gram-positive bacteria. INTRODUCTIONToday, biological methods of nanoparticle synthesis using microorganisms, natural extracts, and nutrients have been suggested as suitable alternatives to chemical and physical methods (Das et al., 2013). Researchers have reported that cerium nanoparticles are effective in dealing with oxidative stress and have an antioxidant role, so these nanoparticles can act as a remover of various forms of reactive oxygen in many physiological and biochemical reactions in the body of living organisms (Dhall et al. al., 2017(. Microalgae are the main group of photoautotrophic organisms known as potential sources of secondary metabolites, phenolic compounds, pigments and polysaccharides. Biosynthesis targets algal secondary metabolites as reducing agents for stabilization of nanoparticles (NPs) (Barciela et al., 2022). Metabolites extracted from starfish with low molecular weight have remarkable characteristics. These isolated compounds include steroids, anthroquinones, alkaloids, phospholipids and peptides, which are a rich source of activity against microbes and have many uses in the field of medicine (Baharara et al., 2020). In this study, the antioxidant and antibacterial properties of green cerium oxide nanoparticles synthesized from marine extracts have been investigated. MATERIALS AND METHODS Sargassum ilicifolium macroalgae was collected from the coast of Chabahar and transported to the laboratory. Then the samples were washed several times with distilled water in order to separate the mud and dried in the shade and at a suitable temperature for a week and then ground to powder. Brittle star Ophiocoma scolopendrina was collected from Qeshm Island at the time of low tide and dried with a freeze dryer at -40C and then powdered with an industrial mill. In order to extract and synthesize CeO2NPs nano cerium oxide, 10 grams of powdered each of the samples was added to 100 ml of double distilled water and stirred for 3 to 4 hours at 60 degrees Celsius, then it was collected with filter paper and then Whitman. In an Erlenmeyer flask, 100 ml of 0.05 M of Ce(NO3)3.6H2O and 20 ml of distilled water were added to it and this solution was stirred using a magnetic stirrer until a homogeneous solution was formed (Altaf et al. al., 2021). Then 40 ml of the extract was added to the solution and the final volume was brought to 100 ml with distilled water and the reaction mixture was stirred on a hot magnetic plate with a temperature of 70-80 degrees Celsius for 3-4 hours. The formed particles after sufficient stirring time were centrifuged at 5000 rpm for 10 minutes, the nanoparticles were repeatedly washed with deionized water and dried in an oven at 60 degrees for 6 hours. Then, the obtained product was calcined in the oven at 400°C for 2 hours to produce a yellow powder of cerium oxide (Elahi et al., 2019). In the test of measuring the antioxidant capacity by the DPPH (2,2-diphenyl-1-picrylhydrazyl) radical method, concentrations of 1000, 500, 125, 250, 62.5 and 31.25 μg/ml were made from 10 mg/ml stock, from 100 μl of each concentration was taken and poured into 96-well plates in triplicate. Also, 100 microliters of 0.3 mM DPPH solution (394.3 g/mol) was added. The 96-well plates were placed in the dark for 30 minutes, and the absorbance of the samples was read by an Elizarider device at a wavelength of 517 nm. RESULTS In the FT-IR study, the peaks around 1417, 1575, and 1577, 1432 cm2 in samples A and B are related to carbonate compounds. Scanning electron microscopy (SEM) has shown that the nanoparticles are spherical and tend to aggregate.The highest reduction rate against ascorbic acid was observed in Sargassum macroalgae at a concentration of 500 μg/ml. DISCUSSION AND CONCLUSION The highest inhibitory activity of NPS synthesized for S. ilicifolium was observed (65.45 ± 0.5% at 1000 μg/ml). The basis of the biological activities of cerium nanoparticles is the Ce3+/Ce4+ surface ratio, the cycle of oxidation and reduction between the Ce3+ and Ce4+ states, which have a unique ability to absorb and release oxygen on their surface and play a key role in antioxidant activity (Dhall and Self, 2018).
علوم زیستی دریا
Razieh Salehpour; Narges Amrollahi Biuki; Mehdi Mohammadi; Aghil Dashtiannasab; pedram ebrahimnejad; ahmad ghasemi
Abstract
The aim of this study was to investigate the different levels of dietary fucoidan on growth performance, survival and biochemical parameters of Litopenaus vannamei. Shrimps with a mean initial weight about 8 g were fed with four different diets containing four levels of fucoidan (0, 0.1%, 0.2% and 0.4%) ...
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The aim of this study was to investigate the different levels of dietary fucoidan on growth performance, survival and biochemical parameters of Litopenaus vannamei. Shrimps with a mean initial weight about 8 g were fed with four different diets containing four levels of fucoidan (0, 0.1%, 0.2% and 0.4%) for 30 days in a completely randomized design with triplicates. The results showed that the growth performance (final weight, weight gain, feed conversion ratio and specific growth rate) of shrimp fed with diet containing fucoidan was significantly (P <0.01) improved compared to shrimps fed with control diet. The best growth performance was observed in shrimps fed diet containing 0.4% fucoidan. Survival did not show a significant difference between the groups fed with fucoidan supplementation and control (P> 0.05). The biochemical parameters of hemolymph including glucose, triglyceride and cholesterol were significantly reduced in shrimps fed with different levels of fucoidan compared to the control group. However, the amount of protein in the groups fed with fucoidan supplementation increased compared to the control. The activity of alanine aminotransferase and aspartate aminotransferase enzymes in hemolymph of control shrimp was higher than shrimps fed with fucoidan diet. In general, the results of the present study revealed that diets containing different levels of fucoidan, especially 0.4%, led to the increment of growth indices and the improvement of hemolymph biochemical parameters, thus improving of the health status of shrimp.
علوم زیستی دریا
Zeinab Tovakol; Gholam-Reza Sharifi-Sirchi; Morteza Yousefzadi
Abstract
Biofoiling is a problem caused by microbial biofilms. Today, many studies have been done to solve the problems of biofouling. The most important studies have been carried out on the identification of natural antibiotic compounds in the marine environment. The most important studies have been performed ...
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Biofoiling is a problem caused by microbial biofilms. Today, many studies have been done to solve the problems of biofouling. The most important studies have been carried out on the identification of natural antibiotic compounds in the marine environment. The most important studies have been performed on the identification of natural antifouling compounds in the marine environment. In this study, the anti-algae properties of organic and aqueous extracts of (Mentha longifora) were investigated using n-hexane, ethyl acetate, methanol and aqueous solutions. Chlorella vulgaris and Chaetoceros muelleri Microalgae, were grown in F2 culture medium. The extracts were dissolved in a certain amount in DMSO solvent and added to test tubes containing microalgae culture medium in different concentrations. After 24 hours, under a light microscope, the number of algae was counted by a neobar slide. Data were analyzed by SAS program and data were compared by Duncan's multiple range test. Statistical analysis of n-hexane extracts on C. vulgaris and C. muelleri showed that there was a statistically significant difference between the treatments at the probability level of 0.001. Extract of n-hexane with a concentration of (20 mg ml -1) for the microalgae C. vulgaris and for microalgae C. muelleri, an extract of (10 mg ml -1) for the n-hexane had the highest inhibitory.
علوم زیستی دریا
Elham Movahednia; Majid Moradi; Amir Houshang Bahri; Ali Motamedzadegan
Abstract
The viscera of yellowfin tuna (Thunnus albacores) protein hydrolysis was produced using enzymes Flavourzim. Hydrolytic conditions (time, temperature, enzyme to substrate ratio) using response surface methodology (Response Surface Method; RSM) and central composite design (Central Composite Design was ...
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The viscera of yellowfin tuna (Thunnus albacores) protein hydrolysis was produced using enzymes Flavourzim. Hydrolytic conditions (time, temperature, enzyme to substrate ratio) using response surface methodology (Response Surface Method; RSM) and central composite design (Central Composite Design was optimization). Through these methods, the effects of three factors, temperature, time and amount of enzyme (independent variable) on the degree Hydrolysis as response surface can be studied. The optimized conditions were the ratio of enzyme to substrate of 11/1 Anson units per gram of protein, time 105 min, temperature 93/44 ° C, which brought about the temperature Hydrolysis to 37/23 %. Based on this study which showed that Hydrolysis proteins contain high amounts of protein (45/67 %), it can be concluded that the model predictions are in good condition and protein hydrolysates can be used in fish diets and as additives in the food industry.
علوم زیستی دریا
Mozhgan Toodooei; Shahla Roozbehani; Ali Noori; Somaye Azadbakht; Hadis Karimifar
Abstract
The safflower (Carthamus tinctorius) belongs to the Compositae family. This plant is widely used in food industries and medicine. The aim of this paper is studying the effect of Safflower extract on physiological sex determination and Masculinization in Guppy fish (Poecilia reticulata). 88 one-week-old ...
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The safflower (Carthamus tinctorius) belongs to the Compositae family. This plant is widely used in food industries and medicine. The aim of this paper is studying the effect of Safflower extract on physiological sex determination and Masculinization in Guppy fish (Poecilia reticulata). 88 one-week-old Guppy fishes were divided into four groups (22 fishes in each group): A, B, C and control (without extract). The fishes were exposed to 50, 100 and 150 ppm concentrations of safflower extract for one month. After 3 months, gonads tissue sections of the fishes were prepared. Tissue changes were examined and compared with the control group that did not receive any extract. Results showed a significant difference in the number of primary spermatocytes in all groups; So the greatest increase of cells was in fishes that receiving concentrations of 100 and 150 ppm extract (p<0.05). The results also showed; with increasing concentration of safflower extract, the number of female follicles decreases significantly (p<0.05). Finally, the results of this study showed; safflower extract increases spermatogenesis and by creating tissue changes, it can induce the sex of guppy fish in the direction of masculinization.
علوم زیستی دریا
Narges Alirahimi; Alaeddin Kordenaeej; Ayatollah Rezaei; Daryush Talei
Abstract
To evaluate the production of beta-carotene, traits and patterns of protein band in D. salina microalgae in the presence of colchicine, after microalgae cultivation, colchicine treatments randomized design concentrations of 0, 0.05 and 0.1 and 0.2% Volumetric weight was applied. Changes in growth and ...
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To evaluate the production of beta-carotene, traits and patterns of protein band in D. salina microalgae in the presence of colchicine, after microalgae cultivation, colchicine treatments randomized design concentrations of 0, 0.05 and 0.1 and 0.2% Volumetric weight was applied. Changes in growth and beta-carotene, chlorophyll A, B and total and carotenoids produced by spectrophotometer measurements, as well as electrophoresis on SDS-PAGE analysis was used to investigate the protein patterns. The results showed that concentrations of colchinicine did not result in significant differences in protein pattern in 11 samples and it just affacted the production of certain proteins.. While this treatment causes a difference in beta-carotene, chlorophyll A, B, total carotenoids and growth. Beta-carotene is the most important metabolites increased in all concentrations of colchicine. Treatment increased the carotenoids, chlorophyll A, B, and total while reduced growth. So the treatment of colchicine reduced the growth and increase the production of beta-carotene, chlorophyll A, B, total and carotenoids is in Dunaliella. While no significant effect on the pattern of protein expression. Therefore , it can be concluded that using treatment of colchicine will be useful in beta-carotene productions.
علوم زیستی دریا
Fatemeh Elmi; Sekineh Khazaee Pool; Maryam Mitra Elmi
Abstract
Mineralized collagen has diverse biological advantages, biocompatibility and biodegradability. In this study, the process of mineralization of the protein collagen type I extracted from the scales of white fish (Rutilus Frisii Kutum), was investigated in the Simulated Body Fluid (SBF). During immersion ...
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Mineralized collagen has diverse biological advantages, biocompatibility and biodegradability. In this study, the process of mineralization of the protein collagen type I extracted from the scales of white fish (Rutilus Frisii Kutum), was investigated in the Simulated Body Fluid (SBF). During immersion in SBF, tricalcium phosphate crystals were precipitated into collagen nanofibrils. SDS-PAGE electrophoresis confirmed the presence of type I collagen, including 3 spiral strands. Infrared Fourier Transform (FTIR) spectroscopy technique was used to characterize extracted collagen nanofibrils and mineralazied collagen. The presence of 60% of minerals in the mineralized Acid Soluble Collagen (ASC) was confirmed by TGA. DSC was used to determine the denaturation temperature of mineralized ASC at 147.5 °C, indicating a higher thermal stability of mineralized collagen. TEM images showed irregular and dense formation of tricalcium phosphate nanoparticles on collagen nanofibrils. Mineralized collagen protein can provide new insight into producing porous scaffolds for tissue engineering, dental implants, and providing medical equipment.
علوم زیستی دریا
Razieh Nasrolahzadeh; Alireza Safahieh; Hossein Zolgharnein; Issac Zamani; Kamal Ghanemi
Abstract
Bisphenol-A (BPA) is one of the most important endocrine disrupters that has arrived to the environment, because of this reason, it has been developed into a detrimental material for human beings and other organisams. There are several ways for degradation or removal of industrial pollutants, ...
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Bisphenol-A (BPA) is one of the most important endocrine disrupters that has arrived to the environment, because of this reason, it has been developed into a detrimental material for human beings and other organisams. There are several ways for degradation or removal of industrial pollutants, in which the bacterial communities plays a major role in the biodegradation of BPA in the environment.In this study, contaminated sediments were collected in Mousa Creek. The isolates were identified by biochemical tests and 16S rRNA gene sequence analysis. The isolated Bacteri was Pseudomonas putida. Growth and degradation ability of isolated baccteri was measured in 100, 200 and 300 ppm of BPA in 24-hour intervals for 6 days. Bacterial growth rate was detected with spectrophotometer at 600 nm. Efficiency of BPA degradation was performed by Liquid-liquid extraction method and measured by HPLC. This bacteri was able to grow at concentrations of 100, 200 and 300 ppm of BPA. So, the biodegradation results from mineral salt medium (MSM) indicated that Pseudomonas putida have the best degradation efficiency at the 200 ppm of the BPA. Biodegredation result by HPLC method show that the percentage of the degradation efficiency at the 200 ppm was 82.8 percent.These results demonstrated this indigenous bacteria (Pseudomonas putida) can be used to improve the bacterial communities contaminated by Bisphenol A and to eliminate the pollutants from the costal environment.
علوم زیستی دریا
Hamid Ramezani Aval Reiabi; Mehrdad Azin; Ali Sheykhinejad
Abstract
Increased population growth and the creation of major economic powers has led to increase in the world's energy consumption. In 2008, 88% of the energy consumption of the world was formed by fossil fuels. Since in Iran, fuel for transportation system produces the essential pollutants, substituting these ...
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Increased population growth and the creation of major economic powers has led to increase in the world's energy consumption. In 2008, 88% of the energy consumption of the world was formed by fossil fuels. Since in Iran, fuel for transportation system produces the essential pollutants, substituting these fuels with biofuels, including bioethanol and biodiesel, can effectively reduce pollution in the environment. Some species of microalgae, have the ability to store high levels of carbohydrates, rather than lipids. These microalgae are good candidates for the production of bioethanol since the carbohydrates in their biomass, can be extracted and fermented to bioethanol. The purpose of this study was to optimize the microalgae culture, to increase the production of starch. This study was performed in laboratory scale. The microalga Chlorella vulgaris was procured from Persian Type Culture Collection (PTCC) of Iranian Research Organization for Science and Technology. Culture media BBM and Z8 were selected for growth and production of starch. Optical density, cell counting and dry biomass were measured in these media. Regarding significant difference (P
علوم زیستی دریا
Masoomeh Nateghzadeh; Soheila Matroodi; Kamahldin Haghbeen
Abstract
Antioxidant used for chronic disease as treatment and for repalcing artificial antioxidant with natural antioxidant; To investigate antioxidant activity, we used marine actinomycetes as an important resources of bioactive compounds.. In this study, for phylogenetic analysis of 15 marine actinomycetes ...
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Antioxidant used for chronic disease as treatment and for repalcing artificial antioxidant with natural antioxidant; To investigate antioxidant activity, we used marine actinomycetes as an important resources of bioactive compounds.. In this study, for phylogenetic analysis of 15 marine actinomycetes isolated from Deylam intertidal sediments, 16S rDNA sequences were used. In addition, the total phenolic content (TPC) was determined with the Folin-Ciocalteu’s reagent using gallic acid as a standard and the results were expressed as mM of gallic acid. Then, the antioxidant potential of the extracts was determined by analytical methods, ferric reducing antioxidants power (FRAP). The antioxidant potential of the crude extracts exhibited strong ferric reducing power activity at 517nm and the reducing power activity was strongly correlated with the total phenolics content of the isolates. Among the tested isolates, AHA1 (1419 µM) and AMJ1 (608.8 ) showed the hieghest and lowest total phenolic content, respectivly. Moreover AHA3 and AMJ5 (230.66 and 75.57µM) showed the highest and lowest reducing power, respectivly. These strains showed acceptable antioxidant activity and could be good candidates for more studies as nature resources of pharmaceutical antioxidant compound.
علوم زیستی دریا
negar fadian behbahani; foad buazar
Abstract
Recently, there is a growing necessity to develop environmentally friendly nanoparticle synthesis methods .In this study, we have investigated synthesis of magnetic iron nanoparticles using Sargassum ilicifolium alcoholic extract. The synthesis and characterization of magnetic iron nanoparticles were ...
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Recently, there is a growing necessity to develop environmentally friendly nanoparticle synthesis methods .In this study, we have investigated synthesis of magnetic iron nanoparticles using Sargassum ilicifolium alcoholic extract. The synthesis and characterization of magnetic iron nanoparticles were obtained via UV-Vis spectrum , X-ray diffraction(XRD), transmission electron microscopy(TEM) and vibrating sample magnetometry(VSM) respectively. Formation of iron nanoparticle was attended whit change color from green to green brownish. The maximum peak absorption of synthesized iron nanoparticles was observed at 428 nm indicating the reduction of iron ions and formation of iron nanoparticles. According to the acquired analyses, shape of nanoparticles was spherical and face centered cubic(FCC) and the crystal size was 26.73 nm. Moreover, as prepared iron nanoparticles were demonstrated superparamagnetic and soft magnetic properties.
علوم زیستی دریا
khanomnaz ebadi; mandana zarei; ali mohammad sanati
Abstract
The aim of this study was to isolate and molecular identification of associated bacteria in sponges, with potential ability of biodegrading crude oil. Serial dilutions of homogenized Pachychalina sp. mesohyl were cultured in suitable medium for growth of marine microorganism. Obtained colonies were screened ...
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The aim of this study was to isolate and molecular identification of associated bacteria in sponges, with potential ability of biodegrading crude oil. Serial dilutions of homogenized Pachychalina sp. mesohyl were cultured in suitable medium for growth of marine microorganism. Obtained colonies were screened based on emulsification index and growth in medium containing 2% oil. Six strains which showed the highest growth rate and emulsification index were tested for the amount of oil removal in the minimal salt medium. Also the molecular identification was done Based on the 16SrRNA sequence and PCR. Removal of oil based on two methods; dry weight and absorption at 420 nm confirmed each other and both followed the same pattern. Accordingly KE5 and KE8 strains showed the highest degree of oil removing and molecular identification results revealed that they were most similar to strains of Staphylococcus aureus subsp. N31 and Luteimonas terricola BZ92r respectively. Also according to the results of bioinformatics analysis, it seems KE6 and KE7 respectively with 84% and 90% similarity with Exiguobacterium sp. AT1b and Pseudomonas rhodesiae CIP 104664 strains, have considerable potential for further molecular and biochemical studies .
علوم زیستی دریا
N Nozarpour; M.B Nabavi; Ronagh M.T Ronagh; B Archangi; N Sakhai
Abstract
In this study, the molecular characteristics of Chiromantes boulengeri in 2014 from intertidal waters of Arvand River in the vicinity of Minoo island were investigated. So, in October 2014 specimens from intertidal of two area of Minoo island were collected and preserved in 70% ethanol, carried out to ...
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In this study, the molecular characteristics of Chiromantes boulengeri in 2014 from intertidal waters of Arvand River in the vicinity of Minoo island were investigated. So, in October 2014 specimens from intertidal of two area of Minoo island were collected and preserved in 70% ethanol, carried out to the khoramshahr University of Marine Science and Technology laboratory and examined. To fulfill the purpose, To extract the DNA, phenol- chloroform method were used and mitochondrial gene 16S rRNA was sequenced and compare with other species from GenBank NCBI. The morphological and phylogeny sequencing results showed that all specimens belong to Chiromantes boulengeri in the study area. By polymorphism analysis, there are mutation within C. boulengeri from two area of Arvand River and it seems that this species for its survival has adapted with different ecological conditions in the study area.
علوم زیستی دریا
zahra zarei Jeliani; Morteza Yusef Zadi; jelveh sohrabipour; hojjat toiserkani
Abstract
Seaweed is favored seafood in some regions and is also used as feedstock for extracting fine chemicals. The total global seaweed production continues to grow This study investigated the biological activities of n-Hexane, Ethylacetate and Methanol extract of two red marine macro algae (Gracilariopsis ...
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Seaweed is favored seafood in some regions and is also used as feedstock for extracting fine chemicals. The total global seaweed production continues to grow This study investigated the biological activities of n-Hexane, Ethylacetate and Methanol extract of two red marine macro algae (Gracilariopsis persica and Hypnea flagelliformis), collected from the coast of Bandar Abbas, Persian Gulf, Iran. For identification the superior species with biological properties, the tested activities included Carotenoids content, total Phenolic content, total flavonoids content, antioxidant activity at the concentration (3 mg/ml) by ferric reducing power (FRP) and total antioxidant capacity (TAC) assay and at final, antibacterial activity was evaluated. This study revealed that the more effective macro algae extracts by maximum antioxidant capacity: FRP and TAC, were recorded for Ethylacetate extracts. The result showed the highest content of phenolic and flavonoid compounds were recorded for the Methanol extracts of Gp. persica, 45.12±0.01 (mg GA/gr DW) and 2.28±0.007 (mg QE /gr DW), respectively while H. flagelliformis showed the maximum Carotenoid content 17±0.06 (mg 100g-1). In addition, the highest antibacterial activity was recorded for the n-hexane and followed by Ethylacetate extracts. In general comparison, though, according to the results, antioxidant and antibacterial activity of species in this study were calculated less than standard, but could be accounted these seaweeds as safe biological properties and with abundance of them in coastal of Bandar Abbas, could be considered for future applications in medicine and dietary supplements.
علوم زیستی دریا
Esmat Salimi; hossein zolgharnein; Bita Archangi; Mohammad Taghi Ronagh; Seyyed Ahmad Ghasemi
Abstract
In this study, two populations of Aphanius ginaonis (Holly, 1929) and Aphanius dispar (Ruppell, 1828) were examined to determine genetic diversity using PCR-RFLP technique. A total of 60 individual specimens were collected from Hormozgan and Bushehr internal waters, comprising two sampling sites; from ...
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In this study, two populations of Aphanius ginaonis (Holly, 1929) and Aphanius dispar (Ruppell, 1828) were examined to determine genetic diversity using PCR-RFLP technique. A total of 60 individual specimens were collected from Hormozgan and Bushehr internal waters, comprising two sampling sites; from Geno hot spring (30 individuals of A. ginaonis) and Mir Ahmad hot spring (30 individuals of A. dispar). The D-loop region of mitochondrial DNA (550 bp) was amplified using PCR followed by RFLP analysis based on 5 restriction endonucleas enzymes (AluI, DpnI, Eco47I, HindIII HinfI). In order to undertake data analysis, Arlequin 3.11 was applied. Results at population levels indicated that Geno individuals have more haplotype diversity than Mir Ahmad individuals (9 and 3 haplotypes respectively). However, further investigation using genetic techniques is required to clarify the molecular history and evolution of Aphanius species in this area. Results obtained from this research would be applicable to understand conservation genetics and management of this important fish species in Iran.
علوم زیستی دریا
Marjan Naseri Karimvand; yadollah nikpour; Ahmad Taghavi Moghadam; Kamal Ghanemi
Abstract
Gelatin is polypeptide with high molecular weight which is derived from collagen of connective tissue, skin, bone and tendons. The most common source of gelatin in the world is skin and bone of cow and pig. Gelatin from persian Gulfs jellyfish Crambionella orsini have been successfully extracted by alkaline ...
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Gelatin is polypeptide with high molecular weight which is derived from collagen of connective tissue, skin, bone and tendons. The most common source of gelatin in the world is skin and bone of cow and pig. Gelatin from persian Gulfs jellyfish Crambionella orsini have been successfully extracted by alkaline extraction.We investigated the proximate composition, gel strength, gelling point, melting point of jellyfish gelatin. Jellyfish gelatin contained 13.1% moisture, 1.3% lipid, 2.4% ash, 78.2% protein. The gelatin showed a gel strength of 33 kPa, a gelling point of 18°C, and melting point of 23°C. The gelatin was composed of α1-chain, α2-chain, β-chain, and γ-chain Jellyfish gelatin did not show superior rheological properties to mammalian gelatin, like other fish gelatin; however, it can be used in various food and cosmetic products not requiring high gel strength.
علوم زیستی دریا
hedayat yavarmoghadam; h zolgharnein; Mohammad Ali Salari Aliabadi; s keyvan; Mohamad Modarresi
Abstract
In this present study genetic diversity of cuttlefish (Sepia pharaonis) populations were investigated using microsatellite markers. Total 51 samples were collected from Bandarabass and Bushehr regions. Tissue sample of arm tips (tentacle) were preserved in 96% ethanol alcohol until using in biotechnology ...
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In this present study genetic diversity of cuttlefish (Sepia pharaonis) populations were investigated using microsatellite markers. Total 51 samples were collected from Bandarabass and Bushehr regions. Tissue sample of arm tips (tentacle) were preserved in 96% ethanol alcohol until using in biotechnology laboratory of Khorramshahr University of Marine Science and Technology. Genomic DNA was extracted with CTAB method. The quality and quantity of extracted DNA was assessed by 1% agarose gel electrophoresis and spectrophotometry, respectively. Polymerase chain reaction conducted with 6 pairs of microsatellite primers. PCR products were electrophoresised on 8% polyacrylamide gel and stained with silver nitrate. These primers were shown 4 pairs of polymorph and 2 pairs of monomorp. Allele Sizes were measured in populations then genetic parameter were calculated using Arlequin and Gen Alex Programs and phylogenetic relationship was determinated and drawn using TFPGA Program. Result obtained showed genetic distance and resemblance distance is 0.282 and 0.754, respectively and genetic differentiation was present 0.031. The findings of present study showed low levels of genetic differentiation but significant between of populations and S. pharaonis were likely two different population. These findings provide useful information for conservation and management of this species in the Persian Gulf.
علوم زیستی دریا
niloofar saki; yadollah nikpour ghanavaty; ahmad taghavi moghadam; kamal ghanemi
Abstract
Jellyfish is one of the poisonous animals that causing human poisoning. Found a variety of jellyfish in the Persian Gulf. Although These species cant Cause of quick death in humans But they have harmful effects on human health system and have Following are the side effects. In this study extracted Crambionella ...
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Jellyfish is one of the poisonous animals that causing human poisoning. Found a variety of jellyfish in the Persian Gulf. Although These species cant Cause of quick death in humans But they have harmful effects on human health system and have Following are the side effects. In this study extracted Crambionella Orsini Jellyfish Venom According to Bloom method and was obtained its Concentration by Biuret method and Calculated LD50 by Jung and Choi method. According to Venom concentration and its LD50 was determined that Cause of death mice 0.5 ml of venom. The use of Na-EDTA for neutralizing venom. This Chelate Was injected In two ways to mice that In both methods, Prevented death. Na-EDTA is dedicated Chelate for Calcium excretion from body that According to nuclear calciums venom is able to separated that from Venom structure and neutralize venom.
علوم زیستی دریا
negin derakhshesh; Abdolali Movahedinia; n s; m h; v b
Abstract
Liver is one of the most important organs in vertebrates that have important roles in detoxifying. The main purpose of the present study was developing appropriate methodology for the primary cultivation of hepatic cells from orange-spotted Grouper, Epinephelus coioides. In present study, hepatocytes ...
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Liver is one of the most important organs in vertebrates that have important roles in detoxifying. The main purpose of the present study was developing appropriate methodology for the primary cultivation of hepatic cells from orange-spotted Grouper, Epinephelus coioides. In present study, hepatocytes were isolated from five grouper individuals. Initially, the fish wiped with 70% ethanol. Liver were removed and cut into small pieces with scissors and hepatocytes were disconnected using different enzymatic digestion with collagenase (Type 1 and 4) and trypsin and additional nutrient materials in culturing mediums. Then, cells were cultured for 2 weeks in Lebowitz L-15 under 3 methods: 1. Using enzymatic digestion by trypsin, 2. Using enzymatic digestion by collagenase (type 1 and 4) and 3. Using nutrients and additives was cultured. Finally, effects of different incubation temperature (20, 25, 28, 30 and 32 degree of Celsius) and Bovine serum content (0, 10 and 20% and 20%+ITS) on cell growth were estimated. According to the results, digestion by collagenase type 4, resulted in more cell colonization and growth in comparison with other methods.In conclusion, the best culture method for primary hepatocyte from orange-spotted Grouper, Epinephelus coioides., was using ITS+20%FBS under 30 degree of Celsius incubation temprature.
علوم زیستی دریا
M. Parviz; yadollah nikpour; A. Taghavi Moghadam; K. Ghanemi
Abstract
In this paper, the nematocyst venom of jellyfish crambionella orsini were fractionated by size-exclusion and anion-exchange chromatography. crambionella orsini is famous jellyfish in Persian gulf. The results of mentioned methods has been investigated. The crambionella orsini venoms has hemolytic activity, ...
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In this paper, the nematocyst venom of jellyfish crambionella orsini were fractionated by size-exclusion and anion-exchange chromatography. crambionella orsini is famous jellyfish in Persian gulf. The results of mentioned methods has been investigated. The crambionella orsini venoms has hemolytic activity, as the other species.After extraction of nematocyst venom, the crude venom has been partially purification by sephadex G-200 gel filtration and DEAE anion exchange chromatography. Protein elution was monitored by uv detection at 280 nm. For determine the hemolytic fraction, every fraction were injected to 3 mice via the tail vein. Finally, all data from both chromatography methods were compared. The gel filtrations first pick and the first and second pick of anion exchange chromatography showed hemolytic activity.Determination the appropriate method for purification of this venom can help to find a comprehensive method for another marine venom especially jellyfish venoms and eventually may be help to find specific antidote for jellyfish stings of these species.
علوم زیستی دریا
Mehrnoush Norouzi
Abstract
Genetic structure of golden mullet, Liza aurata, investigated in the Gomishan and Anzali wetlands using six microsatellite primer sets (Muce55, Muce37, Muso10, Muco16, Muso19, and Muso22). Totally 60 samples of adult golden mullet were collected from these regions. All primer sets as polymorphic loci ...
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Genetic structure of golden mullet, Liza aurata, investigated in the Gomishan and Anzali wetlands using six microsatellite primer sets (Muce55, Muce37, Muso10, Muco16, Muso19, and Muso22). Totally 60 samples of adult golden mullet were collected from these regions. All primer sets as polymorphic loci were used to analyze the genetic variation. Analyses revealed that average of alleles (Na) per locus was 5.3 (range 3 to 9 alleles) and in regions, samples of Gomishan wetland 5.1 and Anzali wetland 5.5 respectively. All sampled regions contained private alleles. The average estimates of inbreeding coefficients (Fis and Fit) values of 6 microsatellites were positive. The average observed and expected heterozygosity was in Gomishan wetland 0.153 and 0.721 and Anzali wetland 0.328 and 0.747 respectively. Deviations from Hardy-Weinberg equilibrium were in all cases (P
علوم زیستی دریا
Farhad Talebi; Ramin R. Manaffar; Abolghasem Esmaeili Fereidouni; Javad Abdi
Abstract
Recently, due to the high costs and a decrease in producing of Lansy PZ, various researches have paid attention to the baker's yeast (Saccharomyces cerevisiae) as a substitute for Lansy PZ in Artemia culture technologies. In this study, the effects of six feeding regimes: Lansy PZ (as control), enriched ...
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Recently, due to the high costs and a decrease in producing of Lansy PZ, various researches have paid attention to the baker's yeast (Saccharomyces cerevisiae) as a substitute for Lansy PZ in Artemia culture technologies. In this study, the effects of six feeding regimes: Lansy PZ (as control), enriched yeast with HUFA, enriched yeast with HUFA and without mannoproteins in wall cells, yeast without mannoproteins in wall cells, industrial yeast 100 and 50 % feeding regimes were respectively examined on the fatty acid composition of two Artemia species (Artemia urmiana and A. franciscana) at a salinity of 80 ppt and a density of 500 nauplii per liter in laboratory cultures. Results showed that the enrichment of baker’s yeast with HUFA had increasing trend on the EPA and DHA contents of baker yeast (19.11 and 34.51%, respectively). The yeast type also had significant effect on the fatty acid composition of the two species of Artemia. The highest content of HUFA obtained when Artemia fed the Lansy PZ diet. Our results recommended that the Artemia fed with HUFA enriched yeast and enriched yeast with HUFA without mannoproteins in wall cells induced higher contents of essential fatty acid (especially DHA) compared to other treatments. On the basis of the present investigation, the enrichment of Artemia with yeast enriched HUFA can be substitute to Artemia fed with Lanzy PZ.