Document Type : Original Manuscript

Authors

Department of Clinical sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.

Abstract

Regarding expansion of Litopenaeus vanamei culture in Iran, and the necessity to work on its reproduction system, conducting a research on its biological reproduction and sexual properties is of high value and sexual properties. Then in this study morphological properties of L. vanamei's sperm were evaluated using phase contrast microscope, Atomic Force Microscopy technique and Electronic microscope. Wight of testis and spermotophore as well as density and survival rate of sperms were measured in L. vanamei.  For this purpose, 20 mature male L. vanamei (35.37 ±4.3) were transferred from a local shrimp hatchery to laboratory. Biometrical indices as well as reproductive properties of shrimp (density, diameter and size of sperms, spermatophore weight and melanisation) were measured. Correlation coefficient between all indices were measured too. Results showed that testis and spermatophore weight were 0.18±o.01g and 0.08±0.01g respectively. Sperm count, survival rate and diameter of sperms were 41.19±3.83 per µl, %69.69±%2.39 and 39.36±10.8 respectively. The highest correlation coefficient was seen between shrimp weight and testis weight (R=0.958), sperm diameter (R=0.91) and sperm survival (R=0.716) respectively, although there was no significant correlation between abnormal sperm or melanized spermatophores and other biometrical indices. 

Keywords

Main Subjects

اسلامبولچی، ش. (1378) ؛ تخمین تراکم اسپرم ماهی کپور معمولی، آمور وازونبرون با استفاده از روش اسپکتروفتومتری. پایان نامه کارشناسی ارشد شیلات، دانشکده شیلات و محیط زیست، دا نشگاه علوم کشاورزی و منابع طبیعی گرگان.
جلالی جعفری، ب.، برزگر دولت آبادی، م. (1389). مدیریت بهداشتی پرورش میگو. چاپ دوم. 256 صفحه. صص 16-17.
مجیدی نسب، الف. (1377). بیماری های میگو های پرورشی. انتشارات نوربخش، جلد اول.
متین فر، ع.، رمضانی فرد، الف. و حقوقی پور، م. (1386). بررسی اثرات درجه حرارت و شوری های مختلف بر رشد و بازماندگی میگوی جوان پاسفید ، مجله پژوهش و سازندگی ، امور دام و آبزیان ، شماره 77، صص 104-96.
Afsharnasab, M., Dashtiannasab, A., Yeganeh,V., 2005. Assessing pathogenesis of the White Spot Syndrome Virus (WSSV) in Whiteleged shrimp. IRAN J FISH SCI, 16, 1, 1-8.
Alfaro J, X. 1993a, Development and deterioration of spermatophores in pond-reared Penaeus vannamei. Journal of the World Aquaculture Society, 1993, 24, 522-529.
Alfaro J, Reproductive quality evaluation of male Penaeus stylirostris from a grow-out pond. J. World Aquac. Soc., 1993b, 24, 6-11.
Amrit, N., Bart, Sudarhma C., Dhirendra P. T, 2006. Spermatophor cryopreservation and artificial insemination of black tiger shrimp, penaus monodon. Aquaculture Res, 37, 523-528.
Andre, L. B., Cintia L.N, juscilaine G.M., Elton P.C. and Wasielesky j. r, 2010. Spermatophore quality of the pink shrimp Fartepenaeus paulensis (Decapoda Dendrobrachiata) broodstock fed with different maturation diets. Aquaculture 307, 34-48.
Ceballos, B. P., Rosas. C. and Racotta. I. S., 2004. Sperm quality in relation eoage and weight of White shrimp Litopenaeus vannamei, Aquaculture, 228, 141-151.
Ceballos-Vázquez, B.P., Aparicio-Simón, B., Palacios, E., Racotta, I.S., 2003. Sperm quality over consecutive spermatophore regeneration in the Pacific white shrimp Litopenaeus vannamei. J. World Aquac. Soc. 35 (2), 178–188.
Dougherty, W.J., Dougherty, M.M., 1989. Electron microscopical and histochemical observation on melanized sperm and spermatophores of pond-cultured shrimp, Peneaeous vannamei, J Inverteb Pathol, 54, 331-343
FAO (Food and Agriculture Organization), 2010. The state of world fisheries and aquaculture. Rome: FAO; Annual report.
Jeyalectumie C, Subramoniam T., 1989. Cryopreservation of spermatophores and seminal plasma of the crab Scylla serrata. Biol Bull.; 177: 247-253.
Klaus, S., Schubart, C.D., Brandis, D., 2009. Ultrastructure of spermatozoa and spermatophores of old world freshwater crabs (Brachyura: Potamidea: Gecarcinucidae, Potamidae, and Potamonautidae). J. Morpholo, 270, 175–193.
Leung-Trujillo J.R., A. L. Lawrcncc, 1987. Observations on the decline in sperm quality of Penaeous setiferous under laboratory conditions. Aquaculture, 35, 363-370.
Liley, N.R., Tamkee,P., Tsai, R. and Hoysak, D.J., 2002. Fertilization and dynamics in rainbow trout: Effects of male age, social experience and spermconcentration and motility on in vitro fertilization. : CAN J FISH AQUAT SC, Volume 59, 1, 144-152(9).
Meunpol, O., Meejing, P., Piyatiratitivorakul, S., 2005. Maturation diet based on fatty acid content for male Penaeus monodon (Fabricius) broodstock. Aquac. Res. 36, 1216–1225.
Narahari, J., 2001. Determination of the ultrastructural pathology of human sperm by Atomic Force Microscopy, Fertility Sterility, 75, 5, 1-12.
Niksirat, H., Kouba. A., Psenicka. M., Kuklina, L., 2013. Ultrastructure of spermatozoa from three genera of crayfish Orconectes, Procambarus and Astacus (Decapoda: Astacoidea): New findings and comparisons, Zoologischer Anzeiger 252 (2013) 226–233. 
Peixoto, S., Cavalli, R.O., Wasielesky, W., D’Incao, F., Krummenauer, D., Milach, A.M., 2004. Effects on age and size on reproductive performance of captive Farfantepenaeus paulensis broodstock. Aquaculture 238, 173–182.
Peixoto, S., Cavalli, R.O., Wasielesky, W., D'Incao, F., Krummenauer, D., Milach, A.M., 2004. Effects of age and size on reproductive performance of captive Farfantepenaeus paulensis broodstock. Aquaculture 238, 173–182.
Peixoto, S., Wasielesky, W.J., Louzada, L.J., 2003. Comparative analysis of pink shrimp, Farfantepenaeus paulensis, and Pacific white shrimp, Litopenaeus vannamei, culture in extreme Southern Brazil. J. Appl. Aquac. 14, 101–112.
Perez-Velazquez, M., Bray, W.A., Lawrence, A.L., Gatlin, D.M., González-Félix, M.L., 2001. Effect of temperature on sperm quality of captive Litopenaeus vannamei broodstock. Aquaculture 198, 209–218
Pratoomchat, B., Piyatiratitivorakul, S., Menasveta, P., Fast, A.W., 1993. Sperm quality of pond-reared and wild caught Penaeus monodon in Thailand. J. World Aquac. Soc. 24, 530– 540.
Racotta, I.S., Palacios, E., Ibarra, A.M., 2003. Shrimp larval quality in relation to broodstock condition. Aquaculture 227, 107–130.
Rosas, C., Sanchez, A., Chimal, M.A.E., Saldana, G., Ramos, L., Sota, L.A., 1993. The effect of electrical stimulation on spermatophore regeneration in white shrimp Penaeus setiferus. Aquat. Living Resour. 6, 139–144.
Sanchez, A., Pascual, C., Sanchez, A., Vargas-Albores., F. and Moullac, G.L. 2001. Hemolymph metabolic variable and immune response in Litopenaeus setiferus adult males: the effect of acclimation .Aquaculture 198, 13-28.
Tsai, S. and Lin, C., 2012. Advantages and Applications of Cryopreservation in Fisheries Science. Can.J. Fish. Aquat. Sci. 59:144-15.