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Study on population diversity of the Black- lippped Oyster (Pinctada persica) using PCR-RFLP methods in the khark, Shidvar, Hendurabi and Larak Island

Document Type : Original Manuscript

Authors

1 Department of Marine Biology, Faculty of Marine and Oceanic Sciences, Khorramshahr University of Marine Science and Technology, Khorramshahr

2 Faculty of Marine Biology. Persian Gulf University, Bushehr

Abstract

In this study, genetic diversity of Pinctada persica population was evaluated using PCR-RFLP method. To determine and compare genetic diversity of P. persica populations in khark, shidvar, hendurabi and larak islands, 51 samples were collected from 1-6 m depths. DNA of samples was extracted by CTAB method. A pair of primers of Pinctada persica was used for polymerase chain reaction. Enzymatic digestion of PCR yield was performed using, AvaΙΙ, DdeΙ, HindIII, SfaNΙ and TaqΙ restriction enzymes. DNAs were collected and stained with silver staining after subjected to poly acrylamide gel electrophoresis The results revealed that electrophoresis and enzymatic digestion fragments of the 51 samples collected from four stations in the northern coast of Persian Gulf with all 5 enzymes were the same The results of this study confirm that Pinctada persica Based on the studied gene is completely homogenous in these stations and show no genetic diversity.

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References
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Journal of Marine Science and Technology
Volume 19, Issue 2 - Serial Number 2
August 2020
Pages 1-10
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