Ahmad Imani; Mehrdad Farhangi; Gholamreza Rafiee; Razieh Yazdanparast; Isabel Vasquez Baanante
Volume 11, Issue 3 , November 2012, Pages 4-15
Abstract
We collected 69 samples of Mudskipper from dominant spices periophthalmus waltoni for genetic study from Hendijan, Khore Zangi and Delvar. A specimen of fin was fixed in Ethanol alcohol (96%) and moved to Biotechnology lab in center study of Persian Golf in Bushehr. Total genomic DNA was extracted by ...
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We collected 69 samples of Mudskipper from dominant spices periophthalmus waltoni for genetic study from Hendijan, Khore Zangi and Delvar. A specimen of fin was fixed in Ethanol alcohol (96%) and moved to Biotechnology lab in center study of Persian Golf in Bushehr. Total genomic DNA was extracted by proteinase-K digestion, phenol: chloroform: isoamyl alcohol extraction and ethanol precipitation. The quality and quantity of DNA was assessed using 1% agarose gel electrophoresis and spectrophotomeric method. Polymerase chain reactions (PCR) were conducted on the target DNA using 6 primers RAPD. PCR products were separated by electrophoresis on polyacrylamid gels (8%) that were stained using silver nitrate. Allele frequency, the effective and real allele, expected and observed heterozygosis, genetic diversity, Shannon Information index, Gst and gene based on analysis of molecular variance (AMOVA) were calculated using the GENALEX and Pop Gene software. The average of Shannon index for Hendijan, Khore zangi and Delvar was respectively 0.3887, 0.2860 and 0.3509. Highest of Gst observed between Hendijan and Khore zangi (0.260) and lowest between Khore zangi and Delvar (0.195). Highest of gene flow (Nm) was observed between Delvar and Khore zangi (1.033) and lowest between Hendijan and Khore zangi (0.687). Highest of Genetic identity observed between Delvar and Khore zangi (0.924) but lowest was between Hendijan and Khore zangi community (0.833). Eventually this study show that three community of our research are separate and belong to two clusters.
Farokh Parafkandeh Haghighi; Farhad Kaymaram
Volume 11, Issue 3 , November 2012, Pages 16-24
Hooman Pourkhosrow; Mazyar Yahyavi; Amir Hoshang Bahri; Hojatollah Forooghifard; Nasim Najmi
Volume 11, Issue 3 , November 2012, Pages 22-30
Abstract
This study was carried out to determine the influence of highly unsaturated fatty acid (EPA, DHA) enriched Artemianauplii on growth and survival of postlarvae of Litopeneausvannamei(PL-1 to PL-15) in Persian Gulf and Oman sea ecology research center of Bandar Abbas. A complete randomized design with ...
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This study was carried out to determine the influence of highly unsaturated fatty acid (EPA, DHA) enriched Artemianauplii on growth and survival of postlarvae of Litopeneausvannamei(PL-1 to PL-15) in Persian Gulf and Oman sea ecology research center of Bandar Abbas. A complete randomized design with 5 treatments and 3 replicates using15 tanks containing 20 liters water each was chosen. The Artemianaupliiwere enriched with (0 to 4%) cod liver oil (treatments A to E) and the larvae were fed 6 times per day at 4 hours intervals. The postlarvae of treatment D which fed with Artemianauplii, 3% of enrichment significantly increased compared to other treatments (p<0.05).The highest survival rate regarding treatment E postlarvae which fed with Artemianauplii, 4% enrichment However with treatment D (3%) and C (2%) no significant difference (p>0.05) was found. The postlarvae of treatment control (A) which was fed with Artemianauplii that it was not enriched, had the lowest growth and survival rate.The present study demonstrated that the highly unsaturated fatty acid enriched Artemianaupliihas positive effect on enhancement of growth performance and survival rate of white leg shrimp postlarvae.
Kamran Lari; Majid Abrehdary
Volume 11, Issue 3 , November 2012, Pages 31-41
Abstract
Changes and Countries development and need to Scientific, Cultural and Industrial commutation at Global level requires accessibility to maps and spatial information from the entire world. Achieving to this aim requires equalization of horizontal and vertical datum of all countries. Nowadays with developments ...
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Changes and Countries development and need to Scientific, Cultural and Industrial commutation at Global level requires accessibility to maps and spatial information from the entire world. Achieving to this aim requires equalization of horizontal and vertical datum of all countries. Nowadays with developments in technology and after that increasing in precision of vertical datums is one of the basic arguments and there have been efforts to find vertical datum and relations between them. One of the problems that exist in using absolute heights is difference between M.S.L and Geoid.
The theoretical basis the absolute heights is Geoid but in practice M.S.L is used as absolute vertical datums. Therefore difference between M.S.L and Geoid is called sea surface topography.
In this research one of the all, we have carried out modeling mean sea level using T/P and Jason-1 satellites altimetry data on Persian Gulf and Oman sea , Accordingly Due to the significance of sea level topography in oceanographic studies a new model proposed using the combined satellites altimetry data and the EGM08 global Geoid model in order to determine and equalize the current height datum for Persian Gulf and Oman sea. Therefore, difference between M.S.L derived from T/P and Jason-1 Satellites altimetry data and EGM08 global Geoid model was assessed -0.9 and 1.1 meter respectively
Esmaeil Kazemi; Naser Agh
Volume 11, Issue 3 , November 2012, Pages 42-51
Abstract
Chronic stresses can influence the behavioral and physiological activities of animals and can lead to reduction of resistance against disease and decrease in growth rate and production.Artemianauplii enriched with essential nutrients particularly fatty acids have been used to promote growth, survival ...
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Chronic stresses can influence the behavioral and physiological activities of animals and can lead to reduction of resistance against disease and decrease in growth rate and production.Artemianauplii enriched with essential nutrients particularly fatty acids have been used to promote growth, survival and resistance of different species of aquatic animals to various environmental stresses as well as infectious diseases. In this study we investigated the resistance to environmental stress (temperature, salinity and hypoxi) in larvae of rainbow trout fed Artemianauplii enriched with vegetable oils. The experiment was carried out in 6 triplicate treatments for a period of 10 days. The feeding treatments included: 1) commercial starter feed for trout (Control) 2) Artemianauplii enriched with fish oil, 3) Artemianauplii enriched with sunflower oil, 4) Artemianauplii enriched with canola oil, 5) Artemianauplii enriched with soybean oil and 6) newly hatched nauplli of Artemia. The results showed significant differences in tolerance of fish larvae to high temperature (20 and 25°C), hypoxia stress (5 ppm) and salinity stress (10, 15 and 20 mg.l-1) among different groups. Highest survival to all experimental stresses (except in one case) was observed in larvae fed Artemianauplii enriched with canola oil compared to those fed control diet ( p<0.05), but in most cases not significant with larvae fed Artemianauplii enriched with fish oil.
Abdolali Movahedinia; Zahra Yarahmadi; Sara Rastgar
Volume 11, Issue 3 , November 2012, Pages 52-62
Soad Yaish-Bachari; Hossein Zolgharnein; Mehdi Mohammadi; Mohammad Ali Salari-AliAbadi; Seied Ahmad Ghasemi
Volume 11, Issue 3 , November 2012, Pages 62-70
Abstract
We collected 69 samples of Mudskipper from dominant spices periophthalmus waltoni for genetic study from Hendijan, Khore Zangi and Delvar. A specimen of fin was fixed in Ethanol alcohol (96%) and moved to Biotechnology lab in center study of Persian Golf in Bushehr. Total genomic DNA was extracted by ...
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We collected 69 samples of Mudskipper from dominant spices periophthalmus waltoni for genetic study from Hendijan, Khore Zangi and Delvar. A specimen of fin was fixed in Ethanol alcohol (96%) and moved to Biotechnology lab in center study of Persian Golf in Bushehr. Total genomic DNA was extracted by proteinase-K digestion, phenol: chloroform: isoamyl alcohol extraction and ethanol precipitation. The quality and quantity of DNA was assessed using 1% agarose gel electrophoresis and spectrophotomeric method. Polymerase chain reactions (PCR) were conducted on the target DNA using 6 primers RAPD. PCR products were separated by electrophoresis on polyacrylamid gels (8%) that were stained using silver nitrate. Allele frequency, the effective and real allele, expected and observed heterozygosis, genetic diversity, Shannon Information index, Gst and gene based on analysis of molecular variance (AMOVA) were calculated using the GENALEX and Pop Gene software. The average of Shannon index for Hendijan, Khore zangi and Delvar was respectively 0.3887, 0.2860 and 0.3509. Highest of Gst observed between Hendijan and Khore zangi (0.260) and lowest between Khore zangi and Delvar (0.195). Highest of gene flow (Nm) was observed between Delvar and Khore zangi (1.033) and lowest between Hendijan and Khore zangi (0.687). Highest of Genetic identity observed between Delvar and Khore zangi (0.924) but lowest was between Hendijan and Khore zangi community (0.833). Eventually this study show that three community of our research are separate and belong to two clusters.
AbdolSaeid Abbasi; Moghadam Kiani; seyed Nasser Saeidi; Amir Saeid Nooramin
Volume 11, Issue 3 , November 2012, Pages 70-80
Abstract
Thousands of containers put into and exit from modern container terminals in seaports everyday. Because of increasing usage of containers in international trade and goods transportation, pioneer ports try to improve their productivity by optimum usage of their tools, capabilities, strengths and opportunities. ...
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Thousands of containers put into and exit from modern container terminals in seaports everyday. Because of increasing usage of containers in international trade and goods transportation, pioneer ports try to improve their productivity by optimum usage of their tools, capabilities, strengths and opportunities. It is important for ports to satisfy economic, social and political objectives in a profitable way.
A wide variety of factors affect optimum container operations in sea ports. The basis of this study was ranking of factors and selection of main factors based on Bernardo's decision making method (group multi-attribute by ranking scale), in Bushehr port. Results of this research showed that factors including machinery, building container terminal in Negin Island, containers area, specialized human resources and law and regulation, have more effect in optimum container operation in Bushehr seaport.
matin khaleghi
Volume 11, Issue 3 , November 2012, Pages 81-90
Abstract
This research aimed to study the process of long and location changes of sea star(Astropecten hemprichi) and sea urchin(Diadema setosum) in the coast of Chabahar Gulf in the Oman Sea, IR. during November 2008 to September 2009. The area of study was at the farthest end of south east of Iran in province ...
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This research aimed to study the process of long and location changes of sea star(Astropecten hemprichi) and sea urchin(Diadema setosum) in the coast of Chabahar Gulf in the Oman Sea, IR. during November 2008 to September 2009. The area of study was at the farthest end of south east of Iran in province of Sistan and Balouchestan. Sampling was done bimonthly during low tide from 5 stations, using 1m×1m quadrating randomly. 1885 samples of Echinodermata were collected and studied. Results showed that 32 of them(1.6%) belong to A.hemprichi species and 28(1.4%) samples belong to D. setosum species. The samples then were counted and identified. Then the samples’ abundance, distribution and stability were investigated. The results show that the maximum abundance of A. hemprichi was occurred in September 2009 (1/92±0/76ind.m-2) and The maximum value for D. setosum was shown in November 2008 (3ind.m-2). Investigation of distribution and stability indicators may reveal that A. hemprichi and D. setosum were dispersed more randomly and they may be considered as the rare species .
haleh rahmani; Nader Shabanipour; S. Ali Rozati
Volume 11, Issue 3 , November 2012, Pages 91-100
Abstract
The optomotor response is one of the methods used for measuring zebrafish behavior towards moving objects. The present research has been carried out on zebrafish reaction to moving objects. In the experiment, newly fish matured of both sexes were used. To study the optical power of zebrafish an appropriate ...
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The optomotor response is one of the methods used for measuring zebrafish behavior towards moving objects. The present research has been carried out on zebrafish reaction to moving objects. In the experiment, newly fish matured of both sexes were used. To study the optical power of zebrafish an appropriate apparatus was designed. Experiments were performed with different widths of stripes in black , white (1, 2 , 7 cm) , with width stripes of 1 cm in red - gray , green - gray at different velocities of 2, 3, 4, 7, 10 , 15 rpm. Results of 2-way ANOVA showed significant differences between the various widths, velocities (P<0.05). Evaluation of results showed that at different widths , fish showed the most optomotor response at a velocity of 10 rpm (0.65±0.13) , the lowest response to velocity was shown at 2 rpm (-0.07±0.22) . Furthermore, the best visibility was observed with 1 cm width (0.70±0.13) (P<0.05). Experiment on color vision also showed that at different velocities , various colored strips, there is no significant difference (P>0.05). Nevertheless, the fish demonstrated the most optomotor response to green - gray color (0.10±0.13), a velocity of 10 rpm (0.41±0.13).