نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه شیلات، دانشکده منابع طبیعی، دانشگاه زابل، زابل، ایران

2 گروه شیلات، دانشکده منابع طبیعی دریا، دانشگاه علوم و فنون دریایی خرمشهر، خرمشهر، ایران

3 گروه شیلات، دانشکده منابع طبیعی، دانشگاه خلیج فارس بوشهر، بوشهر، ایران

چکیده

در این پژوهش تأثیر استفاده از چهار سطح نوکلئوتید ( 0، 2/0، 4/0 و 6/0 درصد) جیره غذایی بر رشد و برخی شاخص های بیوشیمیایی همولنف مولد میگوی سفیدغربی (Litopenaeus vannamei) در چهار تیمار آزمایشی (هر یک دارای سه تکرار) بررسی شد.نمونه گیری همولنف طی 3 مرحله یعنی ابتدای دوره (قبل شروع تغذیه)، 21 روز (قبل از قطع پایه چشمی) و 30 روز (9 روز پس از قطع پایه چشمی) پس از شروع تغذیه با جیره های غذایی هر تیمار صورت گرفت. در طی دوره آزمایش دما (24/1± 79/27 درجه سانتیگراد)، اکسیژن محلول (41/0± 78/5 میلی گرم در لیتر)، شوری (1/3± 82/31 ppt) و pH (18/0± 9/7) آب تیمارهای آزمایشی روزانه بررسی و ثبت شد. شاخص های بیوشیمیایی همولنف مولدین آزمایشی از جمله گلوکز، کلسترول، تری گلیسرید، کلسیم، آلبومین، پروتئین کل، HDL و LDL در ابتدای دوره تغذیه ای، 21 روز (قبل از قطع پایه چشمی) و 30 روز پس از شروع تغذیه به روش های متداول بیوشیمیایی مورد سنجش قرار گرفت. نتایج نشان دادند افزایش وزن، درصد افزایش وزن، ضریب رشد ویژه و میزان رشد روزانه مولدین تغذیه شده با تیمارهای مختلف نوکلئوتید نسبت به گروه شاهد اختلاف معنی داری نشان نداد (05/0P>). طبق نتایج، سطوح گلوکز، کلسترول، تری گلیسرید، آلبومین و پروتئین کل همولنف مولدین آزمایشی پس از 21 روز تغذیه با جیره های حاوی نوکلئوتید افزایش معنی داری یافت (05/0P

کلیدواژه‌ها

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